Prokaryotic genetic system contains:
A DNA nucleotide chain has A G C T T C G A sequence the nucleotide sequence of other chain would be
Genetic mutations occur in
Bond between phosphate and sugar in a nucleotide is ..........
DNA replication in lagging strand of the eukaryotes organisms is .......
Conservative and continuous
Sami conservative but continuous
Conservative and semi - discontinuous
Semi conservative but discontinuous
Control of gene expression takes place at the level of
Anti parallel strand in DNA is due to
Genetic code determines the
Protein that accelerates gene expression
Gene that switched other genes on or off
Cluster of structural genes with related function
Protein that suppresses gene expression
A Sequence of how many nucleotides in messager RNA makes a codon for an amino acid?
the following pairs is not correctly matched?Which of
r-RNA - RNA molecules found in ribosomes
ATP - The principal energy carrying compound inthe cell
Purines - Nitrogenous bases Cytosine, Thymine and Uracil
Recombinant DNA - DNA forming by union of segments of DNA from different sources
The first codon to be deciphered was ........... which codes for .............. .
Single nucleotide
Three nucleotides
Two nucleotides
Select the correct sequence of following in DNA replication
Single stranded binding proteins - Helicase - Topoisomerase - DNA polymerase
Helicase - Topoisomerase -DNA polymerase - Single stranded binding proteins
Helicase - single stranded binding proteins -Topoisomerase - DNA polymerase
Helicase - DNA polymerase - Topoisomerase - Single stranded binding proteins
DNA Replication occurs at .......
The following ratio is generally constant for a given species
T + C / G+ A
G + C / A + T
A + G / C + T
Nucleoside is .................
Phosphoric acid + Sugar + base
synthesis of DNA occurs in one strand, because
DNA molecule being synthesised is very long
It is a more efficient process
DNA ligase has to have a role
DNA dependent DNA polymerase catalyses polymerisation only in one direction (5' -->3')
Which one of the following makes use of RNA template to synthesize DNA
DNA dependant RNA polymerase
DNA replication in eukaryotes is ............
Bidirectional with single origin
Unidirectional with single origin
Unidirectional with many origin
Bidirectional with many origin
Regulation of lac operon by repressor is referred to as ..............
Both (1) and (2)
Positive regulation
Nagative regulation
Aminoacids lysine serine and arginine are coded by how many codons?
Assertion: Operon concept was given by Hershey and chase---Reason: Separation of DNA fragments are done by centrifugation technique
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If Assertion is True but the Reason is False
If both Assertion & Reason are false
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
Which of the following enzymes can detect and correct the wrong inserted base during DNA replication?
The direction of DNA replication is ............
From 5' end towards 3' end
From 3' end towards 5' end
Carboxy terminus to amino terminus
Amino terminus to carboxy terminus
Assertion: Mutation results in changes in the genotype and the phenotype of an organism ---Reason: Mutation is a phenomenon, which results in alternation of DNA sequences
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If both Assertion & Reason are false
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If Assertion is True but the Reason is False
Assertion: Replication and transcription occur in the nucleus but translation occurs in the cytoplasm ---Reason: Transcription and translation are unidirectional
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are false
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If Assertion is True but the Reason is False
Which of the following enzymes help in the process of formation of phosphor di-ester bond during reverse transcription?
DNA - dependant DNA polymerase
RNA - dependant DNA polymerase
RNA - dependant RNA polymerase
DNA - dependant RNA polymerase
Which is not correct regarding the activity of helicase during DNA replication?
Stabilizes single strands
State the anticodon of initiation codon of protein synthesis
The code AUG stands for ...............
Assertion: Formation of Lagging strand is slow ---Reason: Formation of lagging strand begins bit later than that of leading strand
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are false
If Assertion is True but the Reason is False
Synthesis of nucleic acids always takes place in
Nitrogen, Hexose sugar and phosphoric acid
Nitrogen base, pentose sugar and phosphoric acid
Nitrogen base, pentose sugar
Nitrogen base, trioses and phoshoric acid
t - RNA attaches amino acid at it's
Which step is not correct in DNA finger printing?
Hybridisation using labelled VNTR probe
Separation of DNA by elecrophoresis
Digestion of DNA by DNA ligase enzyme
Assertion: DNA is cut into Sections by using restriction endonucleases ---Reason: Ligase used to join DNA nucleotides
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If Assertion is True but the Reason is False
If both Assertion & Reason are false
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
Nucleotide arrangement in A can be seen by
The back bone of RNA is consists of which of the following sugar
Ribose
Sucrose
Deoxyribose
Maltose
Transcription begins when one of the following enzymes binds to promotor site.
Regulation of plant hormones
Enhancing photosynthesis in desert plant
Which of the following is used in DNA multiplication?
What does operon contain?
Regulator gene +promotor gene
Operator gene + structural gene
Regulator gene + promotor gene + operator gene + structural gene
Regulator gene + structural gene structural gene
The total number f nitrogenous bases in human genome is estimated to be about
During transcription if the nucleotide sequence of the DNA strand that is being coded is ATACG then the nucleotide sequence in the m RNA would be
Measelson and Stahl's experiment proved
Semi-conservative nature of DNA replication
DNA is the genetic material
DNA is not present in ..........
Chioroplast
Bacteriophage
Mitochondria
A gene containing multiple exons and at least one intron is termed as ...........
0/1
Method of DNA replication in which two strands of DNA separates and synthesize new strands?
Assertion: Helicase is called unwindase ---Reason: DNA helix uncoils and splits into single strands by breaking of hydrogen bonds between complementary bases
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are false
If Assertion is True but the Reason is False
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
Which of the following is not a pyrimidine base?
Assertion: There occur 64 codons in dictionary of genetic code---Reason:Genetic code is quadraplete
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If both Assertion & Reason are false
If Assertion is True but the Reason is False
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
Semi conservative replication of A was first demonstrated in
Number of H - bonds between guanine and cytosine are .......
Okazaki fragments are joined by enzyme
When lactose is present in the culture medium:
Repressor is able to bind to the operator
Both (a) and (b) are correct.
Repressor is unable to bind to the operator
Transcription of lac y, lac z, lac a genes occurs.
DNA acts as a template for synthesis of .........
In which of the following DNA directly involved?
In a transcription unit promotor is said to be located towards
3' end of structural gene
5' end of structural gene
5' end of template strand
3' end of template strand
Pneumococcus experiment proved that
Bacteria undergo binary fission
Bacteria do not reproduce asexually
RNA may sometimes control a production of DNA and protein
Substance common in DNA molecules is made up of
Hexose Sugar
Thymine
Histamine
What is the basis for the difference in the synthesis of the leading and lagging strand of DNA molecules?
0/1
Helicases and single-strand binding proteins that work at the 5' end
DNA polymerase can join new nucleotides only to the 3' end of the growing stand.
DNA ligase works only in the 3' _ 5' direction.
Origin of replication occurs only at the 5' end of the moleecules
The lac opern consists of ...............
One regulatory gene and three structural genes
Two regulatory genes and three structural genes
Three regulrtory genes three structural genes
Four regulatory genes only
The net electric charge on DNA and histones is ........
Assertion: Mutations effect protein structure and functions ---Reason: Only one changed insertion of one amino acid
If both Assertion & Reason are false
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If Assertion is True but the Reason is False
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
Okazaki fragments are synthesized on
Leading strands of DNA only
Complemetary DNA strand
Leading and Lagging strands
Lagging strands of DNA only
What does ''lac'' refer to, in what we call the lac operon?
Assertion: In DNA fingerprinting, after hybridization with VNTR probe, the auto radiogram gives many bands of different sizes. --- Reason: It differs from individual to individual in a population except fraternal twins.
If Assertion is True but the Reason is False
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are false
Assertion: Normally the genetic code is degenerate ---Reason: One amino acid is coded by more than one codon
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If Assertion is True but the Reason is False
If both Assertion & Reason are false
Similarity in DNA and RNA is ............
0/1
The enzyme amino acyl t-RNA -synthetase facilitates
Joining two neighbouring amino on ribosomes
Insertion of amino acyl t-RNA into the ribosome sites.
Adoption of amino acids by a t-RNA molecule
Transfer of amino acyl t-RNA from the ribosomal 'A' site to 'P' site
Which of the following is the correct sequence of event with reference to the central dogma?
Transcription, Translation, Replication
Duplication, Translation, Transcription
Transcription, Replication, Translation
Replication, Transcription, Translation
If one strand of DNA has the nitrogenous base sequence as ATCTG, What would be the complementary RNA strand sequence
In some viruses, DNA is synthesised by using RNA as template. Such a DNA is called as ........
E. coli ell grown on 15N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?
One intermediate density band
One low and one intermediate density band
One high and one low density band
One high and one intermediate density band
Termination of polypeptide chain is brought about by
A functional unit in synthesis of protein is
Hershey and Chase experiment with bacteriophage showed that
DNA is the genetic material
Viruses undergo transformation
DNA contains radioactive sulphur
Protein gets into the bacterial cells
DNA is acidic due to .......
DNA acts as a template for synthesis of ..........
Functioning of structural genes is controlled by ................
Which of the following enzyme is not produced by E.coli during lactose catabolism?
Thio glactoside translacetylase
The approximate number of genes contained in the genome of Kalpana Chawla was
The information from RNA to DNA are transferred by which process?
Transcription
Translation
Replication
DNA polymerase is needed for ......
Whose experiments cracked the DNA and discovered unequivocally that a genetic code is a ''triplet''
The minimum requirement for mutation is
Multiplication of DNA is called as
One - gene - one enzyme hypothesis was proposed by ..................
The change of chromosomal parts between non homologous pairs of chromosome is ...........
The amino acid attaches to the tRNA at it's
DHU loop
S' - end
3' - end
Anti codon site
Codons constitute genetic code because
There was 64 types of amino acid
In the genetic code dictionary how many codons are used to code for all the 20 essential amino-acids?
Which of the following causes mutation
Non - sense codons take part in
Conversion of sense DNA in to non-sense one
Releasing t-RNA from polynucleotide chain
Terminating gene controlled protein synthesis
Formation of unspecified aminoacids
DNA polymerase and ligase
Enzymes needed for formation of replication fork is ..............
RNA polymerase and DNA polymerase I
mRNA molecule is produced by
Heat killed pathogenic cells and live non pathogenic cells are mixed and injected into Mice the result would be
Mice develop disease and die
50% mice develop discease and die
Mice die without developing disease
Termination of chain growth in protein synthesis is brought about by ................
Semionservation replication of DNA was given by ...........
Betason and punnett
Avery, McCarty and Mactleod
Watson and Crick
Messelson and Stahi
Correct sequence of code transfer during polypeptide formation is .......
DNA, mRNA, tRNA and amino acids
m RNA, tRNA, DNA and amino acids
The enzyme involved in transcription is ............
RNA polymerase
DNA polymerase I
DNA polymerase II
Semi conservative DNA replication using 15 N was demonstrated by
Which of the following amino acid is coded by 3 codons?
t-RNA attach to larger sub unit of ribosome with the help of which loop?
Protein synthesis is an animal cell occurs
On ribosomes present in cytoplasm as well as in mitochondria
Only on the ribosomes present in cytosol
Only on ribosomes attached to the nuclear envelop and endoplasmic reticulum
On ribosomes present in the nucleolus as well as in cytoplasm
The first genetic material could be
Carbohydrates
Protein
DNA
Select correct match with respect to lac-operon model
Active represser + inducer = Inactive repressor
Inactive repressor + corepressor = Active repressor
Active repressor + corepressor = Inactive repressor
Inactive repressor + inducer = Active repressor
Which one of the following triplet codes, is correctly matched with its specificity for an amino acid in protein synthesis or as 'start' or 'stop' codon?
DNA and RNA are similar with respect to
The same sequence of nucleotides for the amino acid phenyl alanine
Thymine as a nitrogen base
A single-stranded helix shape
Nucleotide containing sugars, nitrogen bases and phosphates
Amino acid sequence in protein synthesis is decided by the sequence of
Intiation codon of protein synthesis (in eukaryotes) is ........
Which of the following statements is not true about DNA replication in eukaryotes?
There are numerous different bacterial chromosomes, with replication ocurring in each at the same time.
Replication is bidirectional from the origins.
Replication occurs at about 1 million base pairs per minute.
Replication begins at a single origin of replication.
Which of the following statements about DNA replication is not correct?
Process is known as semi conservative replication one old strand is conserved in the new molecule.
Complementary base pairs are held together with hydrogen bonds
Replication occurs as each base is paired with another exactly like it
Unwinding of DNA molecule occurs as hydrogen bonds break.
Genetic information are transferred from nucleus to cytoplasm of cell through
Who proved DNA as a genetic material
Which is the smallest RNA?
Which of the following is not associated with HGP?
Cloning vectors BAC & YAC
A Sequence of three consecutiue bases in a t - RNA molecule which Specifically binds to a complementary codon sequence in m - RNA is known as ............
Ribosomal RNA is actively synthesized by .....
Assertion: Lac operation is an inducible system ----Reason: Transcription occurs in the presence of lactose
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are false
If Assertion is True but the Reason is False
Southern blotting is used for the identification of
All the above
RNA
Protein
DNA
The strand of DNA, which does not code for anything is referred to as ............
Antisense strand
Coding strand
Template stand
What is the nature of the 2 strands of a DNA duplex?
Ant parallel & Complimentary
Dissimilar & non complimentary
Anti parallel & non complimentary
Identical & Complimentary
What does a gene consist of?
Translation is the process in which ...........
RNA is formed on DNA template
Protein is formed from RNA
DNA is formed on DNA template
DNA is formed on RNA template
Double helix model of DNA is proposed by
Name the enzymes needed for lactose catabolism in E.coli?
B - galactosidase, lactase, permease
Lactase, permease, transacetylase
B - galactosidasee Lactase, transacetylase
B - galactosidase, permease transacetylase
Method by which information reaches from DNA and RNA is .......
Transformation
Translation
Transcription
Who supported Griffith effect by molecular explanation?
Avery, Mc Carty and Macleod
Both dexoyribose and ribose belong to a class of sugars called as .....
By which bonds the purine & pyrimidine pairs of complementary strands of DNA held together?
C - bonds
O - bonds
H - bonds
Genetic information is carried by the long chain molecules which are made up of ............
One of the following is true with respect to AUG
It codes for methionine only
It is also an initiation codon
It codes for methionine in both prokaryotes and eukaryotes
What is called Griffith effect?
Assertion: Catching criminals could become easier and quicker using DNA fingerprints ---Reason: The process begins with blood or cell sample from which DNA is extracted
If both Assertion & Reason are false
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If Assertion is True but the Reason is False
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
Genetic code was deciphered by chemically synthesizing the trinucleotides by
Waston & Crick
Beadle & Tatum
Briggs & King
Assertion; DNA is a chemically and structurally more stable. ---Reason: DNA have no catalytic properties.
If Assertion is True but the Reason is False
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If both Assertion & Reason are false
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
Which is not the step of translation?
Poly some is formed by ..............
Several ribosomes attached to a single m RNA
Ribosomes attached to each other in a linear arrangement
A ribosome with several sudunits
Many ribosomes attached to a strand of endoplasmic reticulum
Assertion: Base pairing between purine and pyrimidines allows uniforms distance between two strands of the helix. ---Reason: Number of hydrogen bonds between pairing bases are constant.
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion
If Assertion is True but the Reason is False
If both Assertion & Reason are false
If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
Which of the following enzyme is used to join DNA fragments?
The promoter site and the terminator site for transcription are located at
3' (upstream) end and 5' (downstream) end, respectively of the transcription unit
3' (downstream) end and 5' (upstream) end, respectively of the transcription unit
Chargaff's rule is given as